• Catalog Number:

  E011513-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422 (T-G-SP-E-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human. Mouse

• SwissProtNo:

  P21860

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  eIF4B (phospho-Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Serine 422.

• References:

  Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (phospho-Ser422) Antibody (E011513).

Western blot analysis of extract from Hela cells using eIF4B (phospho-Ser422) Antibody (E011513).