• Catalog Number:

  E011511-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eIF2a around the phosphorylation site of Serine 49 (L-L-SP-E-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse ,Rat

• SwissProtNo:

  P05198

• Applications:

  IHC: 1:50~100

• Specificity Sensitivity:

  eIF2α (phospho-Ser49) Antibody detects endogenous levels of eIF2a only when phosphorylated at Serine 49.

• References:

  Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (phospho-Ser49) Antibody (E011511).