• Catalog Number:

  E011331-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CARM1 around the phosphorylation site of serine 228 (V-K-SP-N-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q86X55

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  CARM1 (Phospho-Ser228) antibody detects endogenous levels of CARM1 only when phosphorylated at serine 228.

• References:

  Selma El Messaoudi, et al. (2006) Proc Natl Acad Sci U S A; 103(36): 13351–13356. Marcela Covic, et al. (2005) EMBO J; 24(1): 85–96. Eleni Zika et al. (2005) Proc Natl Acad Sci U S A.; 102(45): 16321–16326 Neelu Yadav et al. (2003) Proc Natl Acad Sci U S A; 100(11): 6464–6468

Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 (Ab-228) antibody (E021331, Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (E011331, Line 3 and 4).