• Catalog Number:

  E011330-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human α-catenin around the phosphorylation site of serine 641 (D-D-SP-D-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P35221

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  α-catenin (Ab-641) antibody detects endogenous levels of α-catenin only when phosphorylated at serine 641.

• References:

  Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765 Drees, F. et al. (2005) Cell 123, 903-915. Yamada, S. et al. (2005) Cell 123, 889-901. Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.

Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 30min), using α-catenin (Ab-641) antibody (E021330, Lane 1 and 2) and α-catenin (Phospho-Ser641) antibody (E011330, Lane 3 and 4).