• Catalog Number:

  E011325-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022

• Applications:

  IHC: 1:50-1:100 WB:1:500-1:1000

• Specificity Sensitivity:

  Smad3 (Phospho-Ser425) Antibody detects endogenous levels of Smad3 only when phosphorylated at serine 425.

• References:

  Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24 Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89 Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200 LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (Phospho-Ser425) Antibody (E011325).