Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human SHP-1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P29350
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
SHP-1 (Phospho-Tyr536) Antibody detects endogenous levels of SHP-1 only when phosphorylated at tyrosine 536.
References:
Migone TS, et al. (1998) Proc Natl Acad Sci USA; 95(7): 3845-3850.
Timms JF, et al.(1998) Mol Cell Biol; 18(7): 3838-3850.
Kanagasundaram V, et al. (1999) Mol Cell Biol; 19(6): 4079-4092.
Hauck CR, et al. (1999) Infect Immun; 67(10): 5490-5494.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SHP-1 (Phospho-Tyr536) Antibody (E011318).