• Catalog Number:

  E011317-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Shc1 around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P29353

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Shc1 (Phospho-Tyr427) antibody detects endogenous levels of Shc1 only when phosphorylated at tyrosine 427.

• References:

  Trampont P, et al. (2006) Mol Cell Biol; 26(23): 9035-9044. Patrussi L, et al. (2005) Oncogene; 24(13): 2218-2228 van der Geer P, et al. (1996) Curr Biol ; 6(11): 1435-1444

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Shc1 (Phospho- Tyr427) antibody (E011317).

Western blot analysis of extracts from 293 cells, using Shc1 (Ab-427) antibody (E021317, Lane 1 and 2) and Shc1 (Phospho-Tyr427) antibody (E011317, Lane 3 and 4).