• Catalog Number:

  E011316-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Shc1 around the phosphorylation site of tyrosine 349 (H-Q-YP-Y-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P29353

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Shc1 (Phospho-Tyr349) antibody detects endogenous levels of Shc1 only when phosphorylated at tyrosine 349.

• References:

  Trampont P, et al. (2006) Mol Cell Biol; 26(23): 9035-9044. Patrussi L, et al. (2005) Oncogene; 24(13): 2218-2228 van der Geer P, et al. (1996) Curr Biol ; 6(11): 1435-1444

Western blot analysis of extracts from 293 cells, using Shc1 (Ab-349) Antibody (E021316, Lane 1, 2 and 3) and Shc1 (Phospho-Tyr349) Antibody (E011316, Lane 4, 5 and 6).