Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PKCζ around the phosphorylation site of threonine 410( T-S-TP-F-C).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q05513
Applications:
IHC: 1:50-1:100
Specificity Sensitivity:
PKCζ (Phospho-Thr410) antibody detects endogenous levels of PKCζ only when phosphorylated at threonine 410.
References:
Parmentier JH, et al. (2004) BMC Cell Biol; 5: 4.
Castrillo A, et al. (2003) Mol Cell Biol; 23(4): 1196-1208.
Paramio JM, et al. ( 2001) Mol Cell Biol; 21(21): 7449-7459.
Braiman L, et al. (2001) Mol Cell Biol; 21(22): 7852-7861.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCζ (Phospho-Thr410) antibody (E011314).