• Catalog Number:

  E011312-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NFκB-p105/p50 around the phosphorylation site of serine 927 (C-D-SP-G-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19838

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  NFκB-p105/p50 (Phospho-Se927) antibody detects endogenous levels of NFκB-p105/p50 only when phosphorylated at serine 927.

• References:

  Hou S, et al. (2003) J Biol Chem. 278(46): 45994-45998. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NFκB-p105/p50 (Phospho-Ser927) antibody (E011312).

Western blot analysis of extract from HT-29 cells, untreated or treated with TNF-α and Calyculin A, using NFκB-p105/p50 (Ab-927) antibody (E021312, Lane 1 and 2) and NFκB-p105/p50 (Phospho-Ser927) antibody (E011312, Lane 3 and 4).