Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-SP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q02078
Applications:
IHC: 1:50-1:100
Specificity Sensitivity:
MEF2A (Phospho-Ser408) antibody detects endogenous levels of MEF2A only when phosphorylated at serine 408.
References:
Okamoto SI, et al. (2002) Proc Natl Acad Sci USA; 99(6): 3974-3979.
Quinn ZA, et al. (2001) Nucleic Acids Res; 29(3): 732-742.
Kato Y, et al. (2000) J Biol Chem. 275(24): 18534-18540.
De Angelis L, et al. (1998) Proc Natl Acad Sci USA; 95(21): 12358-12363.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A (Phospho-Ser408) antibody (E011309).