• Catalog Number:

  E011308-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of threonine 334 (P-Q-TP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49137

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  MAPKAPK-2 (Phospho-Thr334) antibody detects endogenous levels of MAPKAPK-2 only when phosphorylated at threonine 334.

• References:

  Rouse, J. et al. (1994) Cell 78, 1027-1037. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MAPKAPK-2 (Phospho-Thr334) antibody (E011308).

Western blot analysis of extract from HeLa cells treated with UV (20min), using MAPKAPK-2 (Ab-334) antibody (E021308, Lane 1 and 2) and MAPKAPK-2 (Phospho- Thr334) antibody (E011308, Lane 3 and 4).