• Catalog Number:

  E011307-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 74 (L-L-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P05787

• Applications:

  IF:1:100~1:200

• Specificity Sensitivity:

  Keratin 8 (Phospho-Ser74) Antibody detects endogenous levels of Keratin 8 only when phosphorylated at serine 74.

• References:

  Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751

Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Phospho-Ser74) Antibody (E011307, Red).