• Catalog Number:

  E011306-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P05783

• Applications:

  IHC: 1:50-1:100 WB: 1:500-1000

• Specificity Sensitivity:

  Keratin 18 (Phospho-Ser33) antibody detects endogenous levels of Keratin 18 only when phosphorylated at serine 33.

• References:

  Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354. Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613. Moll, R. et al. (1982) Cell 31, 11-24.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (Phospho-Ser33) antibody (E011306). P-Peptide + - -

Western blot analysis of extracts from A431 cells (Lane 1 and 2) and EC-304 cells (Lane 3) using Keratin 18 (Phospho-Ser33) antibody (E011306).