• Catalog Number:

  E011297-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKCθ around the phosphorylation site of serine 676 (R-L-SP-F-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04759

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Alter Name:

  PRKCQ , Protein kinase C theta type

• Specificity Sensitivity:

  PKCθ (phospho-Ser676) antibody detects endogenous levels of PKCθ only when phosphorylated at serine 676.

• References:

  Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253. Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123. Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCθ (phospho- Ser676) antibody (E011297).

Western blot analysis of extract from Jurkat cells untreated or treated with PMA (1ng/ml, 5min), using PKCθ (Ab-676) antibody (E021289, Lane 1 and 2) and PKCθ (phospho- Ser676) antibody (E011297, Lane 3 and 4).