• Catalog Number:

  E011291-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q13480

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Gab1 (phospho-Tyr627) antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 627.

• References:

  Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904. Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307. Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.

Western blot analysis of extracts from HUVEC cells untreated or treated with EGF (200ng/ml, 15min), using Gab1 (Ab-627) antibody (E021283, Lane 1 and 2) and Gab1 (phospho-Tyr627) antibody (E011291, Lane 3 and 4).