• Catalog Number:

  E011286-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human α- Synuclein around the phosphorylation site of tyrosine 136 (Q-D-YP-E-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P37840

• Applications:

  IF:1:100~1:200

• Specificity Sensitivity:

  α-Synuclein (phospho-Tyr136) antibody detects endogenous levels ofα-Synuclein only when phosphorylated at tyrosine 136.

• References:

  Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33 Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42 Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2 Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.

Immunofluorescence staining of methanol-fixed HeLa cells using α-Synuclein (phospho-Tyr136) antibody (E011286, Red).