• Catalog Number:

  E011284-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23443

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100 IF: 1:100-200

• Specificity Sensitivity:

  p70 S6 Kinase (phospho-Ser424) antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at serine 424.

• References:

  Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851 Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c Le, X.F, et al. (2003) Oncogene 22: 484–97

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (phospho-Ser424) antibody (E011284).

Western blot analysis of extracts from 293 cells, untreated Western blot analysis of extracts from 293 cells untreated or treated with serum for the indicated times, using p70 S6 Kinase (phospho-Ser424) antibody (E011284).