• Catalog Number:

  E011279-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eIF2α around the phosphorylation site of serine 51 (E-L-SP-R-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05198

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  eIF2α (phospho-Ser51) antibody detects endogenous levels of eIF2α only when phosphorylated at serine 51.

• References:

  Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460. Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992. Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using eIF2α (phospho-Ser51) antibody (E011279).

Western blot analysis of extracts from K562 cells untreated or treated with IFN-α (100ng/ml, 20min), using eIF2α (Ab-51) antibody (E021271, Lane 1 and 2) and eIF2α (phospho-Ser51) antibody (E011279, Lane 3 and 4).