• Catalog Number:

  E011272-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14247

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  Cortactin (Phospho-Tyr466) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.

• References:

  Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093 Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229 Li Y, et al. (2000) J Biol Chem ; 275(47): 37187-37193 Kinley AW, et al. (2003) Curr Biol ; 13(5): 384-393

Western blot analysis of extracts from Hela cells, untreated or treated with UV (20min), using Cortactin (Ab-466) antibody (E021264, Lane 1 and 2) and Cortactin (Phospho-Tyr466) antibody (E011272, Lane 3 and 4).