• Catalog Number:

  E011251-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NFκB-p105/p50 around the phosphorylation site of serine 932 (E-T-SP-F-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19838

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  NFκB-p105/p50 (phospho-Ser932) antibody detects endogenous levels of NFκB-p105/p50 only when phosphorylated at serine 932.

• References:

  Sören Beinke et al. (2004) Biochem J. 382(Pt 2): 393–409. Vigo Heissmeyer1, et al. (1999) The EMBO Journal 18: 4766–4778, F Chen, et al. (2006) Cell Death and Differentiation 13: 1835–1838. Kris A. Steinbrecher, et al. (2005) Mol Cell Biol. 25(19): 8444–8455.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NFκB-p105/p50 (phospho-Ser932) antibody (E011251).

Western blot analysis of extracts from HeLa cells, untreated or treated with TNFα (20ng/ml 5min) and Calyculin A (50nM 15min), using NFκB p105/p50 (Ab-932) antibody (E021243, Line 1 and 2) and NFκB p105/p50 (phospho-Ser932) antibody (E011251, Line 3 and 4)