• Catalog Number:

  E011249-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human JNK2/3/SAPK around the phosphorylation site of threonine 183 (M-M-TP-P-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P45984; P53779

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  SAPK/JNK (phospho-Thr183) antibody detects endogenous levels of SAPK/JNK only when phosphorylated at threonine 183.

• References:

  I. Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23  Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206 D.Margriet Ouwens1, et al. (2002) The EMBO Journal 21: 3782–3793, S Matsuyoshi1, et al. (2006) British Journal of Cancer 94: 532

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (phospho-Thr183) antibody (E011249).

Western blot analysis of extracts from 293 cell using SAPK/JNK (Ab-183) Antibody (E021241, Lane 1, 2) and SAPK/JNK (phospho-Thr183) antibody (E011249, Lane3, 4).