• Catalog Number:

  E011247-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human,

• SwissProtNo:

  P04792

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  HSP27 (phospho-Ser78) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 78.

• References:

  J Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803 Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301 Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003) Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 (phospho- Ser78) antibody (E011247).

Western blot analysis of extracts from HeLa cells using HSP27 (Ab-78) antibody (E021239, Lane 1 and 2) and HSP27 (phospho-Ser78) antibody (E011247, Lane 3 and 4).