• Catalog Number:

  E011244-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06493

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  CDC2 (phospho-Tyr15) antibody detects endogenous levels of CDC2 only when phosphorylated at tyrosine 15.

• References:

  Y Gu, et al. (1992) EMBO J. 11(11): 3995–4005. P Jin, et al. (1996) Cell Biology, Vol 134: 963-970, Tyagi, et al. (2005) Alpna1Carcinogenesis, Volume 26: 1978-1987(10) Xiang S. et al. (1997) The EMBO Journal 16: 182–192

Western blot analysis of extracts from COLO (Lane 1, 5) and HepG2 (Lane 2, 6) and K562 (Lane 3, 4, 7, 8) cells, using CDC2 (Ab-15) antibody (E021236, Lane 1, 2, 3 and 4) and CDC2 (phospho-Tyr15) antibody (E011244, Lane 5, 6, 7 and 8).