• Catalog Number:

  E011242-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P38398

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  BRCA1 (phospho-Ser1423) antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1423.

• References:

  Kim ST, et al. (2002) Genes Dev; 16(5): 560-570 Arlt MF, et al. (2004) Mol Cell Biol; 24(15): 6701-6709 Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718 Greenberg RA, et al. (2006) Genes Dev; 20(1): 34-46 Tibbetts RS, et al. (2000) Genes Dev; 14(23): 2989-3002

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BRCA1 (phospho-Ser1423) antibody (E011242).

Western blot analysis of extracts from K562 cells treated with UV (20min), using BRCA1 (phospho-Ser1423) antibody (E011242, Lane 1 and 2) .