• Catalog Number:

  E011240-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-kit around the phosphorylation site of tyrosine 721 (N-E-YP-M-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10721

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  c-Kit(Phospho-Tyr721) antibody detects endogenous levels of c-kit only when phosphorylated at tyrosine 721.

• References:

  Nocka, K. et al. (1990) EMBO J. 9, 1805-1813. Hirota, S. et al. (1998) Science 279, 577-580. Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162. Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253. Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.

Western blot analysis of extracts from 293T cells(transfected with wild or mutant type c-kit/pc DNA3.1) treated with SCF(100ng/ml, 15min), using c-Kit(Phospho-Tyr721) antibody (E011240).