• Catalog Number:

  E011229-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 869 (K-E-YP-H-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  EGFR (phospho-Tyr869) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 869.

• References:

  Inoue A, et al. (2005) PLoS Med; 2(1): e13 Sun H, et al. (2004) EMBO J; 23(1): 100-110 Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720 Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674 O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94(7): 3250-3255 Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441

Western blot analysis of extract from A431 cells untreated or treated with EGF (200ng/ml, 5min), using EGFR (Ab-869) antibody (E021222, Lane1 and 2) and EGFR (phospho-Tyr869) antibody (E011229, Lane 3 and 4).