• Catalog Number:

  E011221-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42345

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  mTOR (phospho-Ser2448) antibody detects endogenous levels of mTOR only when phosphorylated at serine 2448.

• References:

  Holz MK, et,al. (2005) J Biol Chem ;280:26089-26093 Chiang GG, et,al. (2005) J Biol Chem ;280: 25485-25490 Mothe-Satney I, et,al. (2004) J Biol Chem ;279: 42628-42637 Bolster DR, et,al. (2003) J Physiol ;553:213-220.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using mTOR (phospho-Ser2448) antibody (E011221).