• Catalog Number:

  E011217-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 529 (L-L-SP-G-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Alter Name:

  NF-KB p65, NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65

• Specificity Sensitivity:

  NF-κB p65 (phospho-Ser529) antibody detects endogenous levels of NF-κB p65 protein only when phosphorylated at serine 529.

• References:

  Xu C, et al (2005) Oncogene:24(28): 4486-95. McNulty SE, et al. (2004) Pigment Cell Res Apr; 17(2): 173-80. Madrid LV,et al. (2001) J Biol Chem: 276(22): 18934-40. Wang D, et al. (2000) J Biol Chem : 275(42): 32592-7.

• All Sites:

  Human: Ser529; Mouse: Ser529; Rat: Ser529

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-κB p65 (Phospho-Ser529) antibody (E011217).

Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-α (20ng/ml, 10min) using NF-κB p65 (Ab-529) antibody (E021210, Line 1 and 2) and NF-κB p65 (phospho-Ser529) antibody (E011217, Line 3 and 4).