• Catalog Number:

  E011215-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05397

• Applications:

  WB:1:500~1000

• Specificity Sensitivity:

  FAK (phospho-Tyr397) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 397.

• References:

  Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613. Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 9021-9026. Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.

Western blot analysis of extract from 3T3 cell, using FAK (phospho-Tyr397) antibody (E011215, Lane 1 and 2).