• Catalog Number:

  E011214-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VASP around the phosphorylation site of serine 157 (R-V-SP-N-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P50552

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Alter Name:

  VASP, Vasodilator-stimulated phosphoprotein

• Specificity Sensitivity:

  VASP (phospho-Ser157) antibody detects endogenous levels of VASP only when phosphorylated at serine 157.

• References:

  Zhao WM, et al. (2001) EMBO J 20(9): 2315-2325. Millard TH, et al. (2005) EMBO J 24(2): 240-250. Kühnel K, et al. (2004) Proc Natl Acad Sci U S A 101(49): 17027-17032. Haffner C, et al. (1995) EMBO J 14(1): 19-27. Ramesh N, et al. (1997) Proc Natl Acad Sci U S A 94(26): 14671-14676.

• All Sites:

  Human: Ser157 or Ser156; Mouse: Ser152; Rat: Ser154

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP (phospho-Ser157) antibody (E011214).

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with forskolin (40μM, 30min), using VASP (Ab-157) antibody (E021207, Lane 1 and 2) and VASP (phospho-Ser157) antibody (E011214, Lane 3 and 4).