Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
Synaptotagmin around the phosphorylation site of serine 309 (G-L-SP-D-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P21579
Applications:
IF:1:100~1:200
Specificity Sensitivity:
Synaptotagmin (Phospho-Ser309) Antibody detects endogenous levels of Synaptotagmin
only when phosphorylated at serine 309.
References:
Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3992-7.
Cnops L, et al. Cereb Cortex. 2008 May; 18(5):1221-31.
Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18(12):4957-68.
Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin (Phospho-Ser309) Antibody (E011209, Red).