• Catalog Number:

  E011206-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p21Cip1 around the phosphorylation site of threonine 145 (R-Q-TP-S-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P38936

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  p21Cip1 (phospho-Thr145) antibody detects endogenous levels of p21Cip1 only when phosphorylated at threonine 145.

• References:

  Ocker M Int,et al. (2007)J Biochem Cell Biol. 39(7-8):1367-74. Dangi S, et al.(2006)Cell Prolif. 39(4):261-79. Chen J,et al.(2006)Am J Physiol Heart Circ Physiol. 290(4):H1575-86

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p21Cip1 (phospho-Thr145) antibody (E011206).