• Catalog Number:

  E011205-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 217 (I-D-SP-M-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02750

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  MEK1 (phospho-Ser217) antibody detects endogenous levels of MEK1 only when phosphorylated at serine 217

• References:

  Shen R, et al. (2002) Mol Cell Biol; 22(10): 3230-3236 Preisinger C, et al. (2005) EMBO J; 24(4): 753-765 Laine P, et al. (2000) Biochem J; 349(Pt 1): 19-25 Yaglom J, et al. (2003) Mol Cell Biol; 23(11): 3813-3824 Dahan S, et al. (2002) Infect Immun; 70(5): 2304-2310

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MEK1 (phospho-Ser217) antibody (E011205).