• Catalog Number:

  E011204-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Raf1 around the phosphorylation site of serine 338 (R-D-SP-S-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P04049

• Applications:

  WB:1:500~1000

• Specificity Sensitivity:

  Raf1 (phospho-Ser338) antibody detects endogenous levels of Raf1 only when phosphorylated at serine 338.

• References:

  Adams DG,et al. (2005)J Biol Chem. 280(52):42644-54. Miura S, et al. (2003) Biochem Biophys Res Commun. 306(4):924-9. Noser JA,et al.(2007)Mol Ther.15(8):1531-6.

Western blot analysis of extracts from MDA-MB-435 cells treated with PMA using Raf1 (phospho-Ser338) antibody (E011204).