恩晶生物-产品-Ezrin (Phospho-Thr566) Antibody

Catalog Number:
E011202-1
Amount:
50ul
Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 566 (Y-K-TP-L-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P15311
Applications:
WB: 1:500~1:1000
Alter Name:
Cytovillin, EZRI, VIL2, Villin 2, p81
Specificity Sensitivity:
Ezrin (phospho-Tyr566) antibody detects endogenous levels of Ezrin only when phosphorylated at threonine 566.
References:
Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91 Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95 Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90 Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9 Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-566) antibody (E021200, Lane 1 and 2) and Ezrin (phospho-Thr566) antibody (E011202, Lane 3 and 4).
All Sites:
Human: Thr566 or Thr567; Mouse:Thr566 or Thr567; Rat: Thr567