• Catalog Number:

  E011201-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 31 (T-P-YP-S-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P49023

• Applications:

  WB: 1:500~1:1000

• Alter Name:

  Paxillin, PAXI, PXN, Paired box protein Pax-1, PAX-1

• Specificity Sensitivity:

  Paxillin (phospho-Tyr31) antibody detects endogenous levels of Paxillin only when phosphorylated at tyrosine 31.

• References:

  Davidson D, et al. (2001) EMBO J 20(13): 3414-3426. Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96(3): 1123-1128. Goldberg MB. (2001) Microbiol Mol Biol Rev 65(4): 595-626. Kook S, et al. (2000) Mol Biol Cell 11(3): 929-939.

• All Sites:

  Human: Tyr31

Western blot analysis of extracts from SK-OV3 cells untreated or estradiol-treated (0.1μM, 20min), using Paxillin (Ab-31) antibody (E021199, Lane 1 and 2) and Paxillin (phospho-Tyr31) antibody (E011201, Lane 3 and 4).