• Catalog Number:

  E011199-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-YP-V-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P11274

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  Bcr (phospho-Tyr177) antibody detects endogenous levels of Bcr only when phosphorylated at tyrosine 177.

• References:

  Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480. Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804. Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695. Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907 – 30916.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue, using Bcr (phospho-Tyr177) antibody (E011199).

Western blot analysis of extract from A431 ce lls, untreated or treated with EGF (200ng/ml, 5min), using Bcr (Ab-177) antibody (E021197, Lane 1 and 2) and Bcr(phospho-Tyr177) antibody (E011199, Lane 3 and 4).