• Catalog Number:

  E011196-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08238

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  HSP90B (Phospho-Ser254) antibody detects endogenous levels of HSP90B only when phosphorylated at serine 254.

• References:

  Zhao R, Houry WA.(2007) Adv Exp Med Biol; 594: 27-36. Olsen JV, et al. (2006) Cell; 127(3): 635-648 Ogiso H, et al. (2004) Biochemistry; 43(49): 15510-15519

Western blot analysis of extract from HT29 cells treated with anisomycin (500ng/ml, 30min), using HSP90B (Phospho-Ser254) antibody (E011196, Lane 1 and2).