• Catalog Number:

  E011185-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9UD71

• Applications:

  IF:1:100~1:200

• Specificity Sensitivity:

  DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.

• References:

  Gammie SC. et al.(2008) PLoS ONE. 2008 Apr 9;3(4):e1974. Alves S. et al. (2008) Hum Mol Genet. 2008 Jul 15;17(14):2071-83. Bibb, J.A. et al. (1999) Nature 402, 669-671 Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155

Immunofluorescence staining of methanol-fixed HeLa cells using DARPP-32 (Phospho-Thr75) Antibody (E011185, Red).