• Catalog Number:

  E011176-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of threonine261 (A-K-TP-R-D) .

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P45985

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  SEK1/MKK4 (phospho-Thr261) antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at threonine 261.

• References:

  Wang W, et al. (2002) Mol Cell Biol; 22(10): 3389-403. Leung IW, et al. (2001) J Biol Chem; 276(3): 1961-7.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SEK1/MKK4(phospho-Thr261)antibody (E011176).