Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PKCθ around the phosphorylation site of serine 695 (N-F-SP-F-M).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q04759
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
PKCθ (phospho-Ser695) antibody detects endogenous levels of PKCθ only when phosphorylated at serine 695.
References:
Xu ZB, et al.(2004) J Biol Chem 279:50401-50409
Thebault S, et al. (2004) Mol Immunol 40: 931-942
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCθ (phospho-Ser695) antibody (E011173).