• Catalog Number:

  E011172-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKCβ around the phosphorylation site of threonine 641 (E-L-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05771

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  PKCβ (phospho-Thr641) antibody detects endogenous levels of PKCβ only when phosphorylated at threonine 641.

• References:

  Zhang Y, et al. (2006) Mol Cell Biol ; 26: 6748-6761 Castoria G, et al. (2004) Mol Cell Biol ; 24: 7643-7653 Marcil J, et al. (1999) Biochem J ; 337:185-192 Bornancin F, et al. (1996) Curr Biol ; 6:1114-1123.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCβ (phospho-Thr641) antibody (E011172).

Western blot analysis of extracts from K562 cells, untreated or treated with PMA (1ng/ml, 10min), using PKCβ (Ab-641) antibody (E021184, Lane 1 and 2) and PKCβ (phospho-Thr641) antibody (E011172, Lane 3 and 4).