• Catalog Number:

  E011168-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NMDAR2B around the phosphorylation site of Tyr1474 (H-V-YP-E-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13224

• Applications:

  IF:1:100~1:200

• Specificity Sensitivity:

  NMDAR2B (phospho-Tyr1474) antibody detects endogenous levels of NMDAR2B only when phosphorylated at Tyr1474.

• References:

  Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895. Westphal, R.S. et al. (1999) Science 285, 93-96. Tingley,W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166. Hisatsune, C. et al. (1997) J. Biol. Chem. 272, 20805-20810.

Immunofluorescence staining of methanol-fixed HeLa cells using NMDAR2B (phospho-Tyr1474) antibody (E011168, Red).