• Catalog Number:

  E011163-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human STAT1 around the phosphorylation site of serine 727 (P-M-SP-P-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42224

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  STAT1 (phospho-Ser727) antibody detects endogenous levels of STAT1 only when phosphorylated at serine 727.

• References:

  Yuan ZM, et al. (1999) Nature.399 (6738): 814-817. Schindler C, et al. (1992) Proc. Natl. Acad. Sci. U.S.A 89:7836-7839 Strausberg R.L (2002). Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903. Quelle F.W., (1995) J. Biol. Chem. 270:20775-20780

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1 (phospho- Ser727) antibody (E011163).

Western blot analysis of extract from thyroid cancer cell line Bph cells (left) and Hela cells (right) , using STAT1 (phospho-Ser727) antibody (E011163).