• Catalog Number:

  E011161-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-SP-F-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02750

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  MEK1 (phospho-Ser221) antibody detects endogenous levels of MEK1 only when phosphorylated at serine 221.

• References:

  Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8. Luciano BS, et al.(2006)J Biol Chem; 279(50): 52117-23. Wang X, et al. (2003) Oncogene; 22(1): 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25. Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (phospho- Ser221) antibody (E011161).

Western blot analysis of extracts from Jurkat cells, using MEK1 (Ab-221) antibody (E021175, Lane 1 and 2) and MEK1 (phospho-Ser221) antibody (E011161, Lane 3 and 4).