• Catalog Number:

  E011160-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-YP-T-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P43403

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  Zap-70 (phospho-Tyr493) antibody detects endogenous levels of Zap-70 only when phosphorylated at tyrosine 493

• References:

  Chan AC, et al. (1995) EMBO J; 14(11): 2499-2508 Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035 Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Zap-70 (phospho-Tyr493) antibody (E011160).