• Catalog Number:

  E011154-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13153

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  PAK1 (phospho-Thr212) antibody detects endogenous levels of PAK1 only when phosphorylated at threonine 212.

• References:

  Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819 Thiel DA, et al. (2002) Curr Biol; 12:1227-1232 Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 (phospho-Thr212) antibody (E011154).

Western blot analysis of extracts from 293 cells, untreated or treated with forskolin (40μM, 30min), using PAK1 (Ab-212) antibody (E021160, Lane 1 and 2) and PAK1 (phospho-Thr212) antibody (E011154, Lane 3 and 4).