Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human TYK2 around the phosphorylation site of tyrosine 1054 (H-E-YP-Y-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P29597
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
TYK2 (phospho-Tyr1054) antibody detects endogenous levels of TYK2 only when phosphorylated at tyrosine 1054.
References:
Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Gauzzi MC, et al. (1996) J Biol Chem. 271(34): 20494-20500.
Western blot analysis of extracts from HT-29 cells untreated or treated with Anisomycin (20min), using TYK2 (phospho-Tyr1054) antibody (E011148, Lane 1 and 2).