Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Lck around the phosphorylation site of tyrosine 393 (N-E-YP-T-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P06239
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Lck (phospho-Tyr393) antibody detects endogenous levels of Lck only when phosphorylated at tyrosine 393.
References:
Heck E, et al. (2006) J Virol. Oct; 80(20): 9934-9942
Michie AM, et al. (2000) Mol Biol Cell. May; 11(5): 1585-1595
Western blot analysis of extracts from MOLT cells using Lck (phospho-Tyr393) antibody (E011144).